小叶黄杨叶降血脂作用研究
1.2 Methods
1.2.1 Division and administrationOne hundred and thirty mice, male and female, weight (25.0 ± 2.0) g, were randomly divided into 13 groups, each with 10, namely: ⑴control group;⑵model group;⑶XueZhiKang group;⑷Water extract high-dose;⑸Water extract low-dose;⑹Petroleum ether extract high-dose;⑺Petroleum ether extract low -dose;⑻Ethyl acetate extract high-dose;⑼Ethyl acetate extract low-dose;⑽Chloroform extract high-dose;⑾Chloroform extract low-dose;⑿n-butyl alcohol extract high-dose;⒀n-butyl alcohol extract low-dose.Sixteen hours after fasting, the mice of group (1)and(2) were given solvent (distilled water including 2% Twain - 80, 0.2 ml/10 g) by gavage; the mice of Group(3) were given Xuezhikang (0.24 g/kg) by intragastric administration; Experimental high-dose treatment groups were gavage corresponding drug 0.90 g / kg; low-dose group were gavage corresponding drug 0.45 g/kg. All above groups delivery capacities were 0.2 ml/10 g. Two hours later, except blank group all the other mice were injected intraperitoneally with 75 percent egg yolk emulsion, dose 0.1 ml/10 g (75 percent egg yolk emulsion: egg yolk from 1-2 d fresh eggs matched with saline ). Six hours later, each group was administered by gastric perfusion corresponding solvent or the medicine once more, the dosage detto, twenty hours after injection of egg-yolk emulsion, blood was extracted from the ophthalmic venous plexus, then blood serum was separated (3 000 RPM per minute, centrifugal 15 minutes). Finally, serum total cholesterol (TC) and triglyceride content (TG) were measured ［6,7,8］.
1.2.2 DeterminationTotal cholesterol was determined by CHOD-PAP; triglycerides was determined by GPO-PAP law operated according to specification.
1.2.3 Statistical treatmentThe measurement data were expressed as mean±standard deviation( ±s)and analyzed by ANOVA with SPSS 1 0.0 software.
2 Result
Serum total cholesterol and triglyceride levels of the model group were higher than those in the control group, the differences were statistically significant (P<0.01), hyperlipidemia model successed.In contrast with the model group, the levels of serum total cholesterol in mice of water extracts of high-and low-dose groups and petroleum ether extracts of high-and low-dose groups and ethyl acetate extracts of high-and low-dose groups were lower,the differences were statistically significant (P<0.01).Compared with the model,the levels of triglyceride in mice of ethyl acetate extracts of high-and low-dose groups were lower than that of model groups, the differences were statistically significant(P<0.01), but the water extracts of high-and low-dose groups and petroleum ether extract high-and low-dose group had no statistically difference (P> 0.05).Compared with the model group, the contents of total cholesterol and triglycerides chloroform extracts of high-and low-dose groups and n-butyl alcohol extracts of high-and low-dose groups had no significant statistical difference (P>0.05). See table 1.Table 1 The effects of the different extracts from Buxus microphylla leaf on TC and TG in hyperlipidemia model mice. （略）
3 Discussion
Hyperlipidemia resulted from lipid metabolism disorder in the body is a common disease in the elderly, frequently-occurring disease,and it is a risk factor leading to arteriosclerotic cardiovascular disease, seriously does harm to human health. With the improvement of living standards, the population of hyperlipidemia is increasing, the demand of human to hypolipidemic drugs is on growing, hence, the development of hypolipidemic agents have good social and economic benefits ［9,10］.A considerable amount of research shows that alkaloids isolated from wood of Buxus microphylla(such as Cyclovirobuxine D) have a better clinical effect on cardiovascular disease .However, the study on the Buxus microphylla leaf is less, and the study on its hypolipidemic isn' t more reported in literatures. In this study the system solvent was used to get different extracts. At the same time their effects on mice hyperlipidemia were observed. The results show that ethyl acetate extracts from Buxus microphylla leaf (0.45-0.90g/kg) significantly reduce level of the serum total cholesterol and triglyceride in hyperlipidemia mice.It provides the experimental data for further study on hyperlipidemic active ingredients and explore Guangxi's rich plant resources.
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